Thermally induced gluten modification observed with rheology and spectroscopies
نویسندگان
چکیده
The protein vital gluten is mainly used for food while interest non-food applications, like biodegradable materials, increases. In general, the structure and functionality of proteins highly dependent on thermal treatments during production or modification. This study presents conformational changes corresponding rheological effects wheat depending temperature. Dry samples analyzed by X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared (FTIR) thermalgravimetric analysis coupled with mass spectrometry (TGA-MS) show surface compositions from 25 to 250 °C. Above 170 °C, XPS reveals a decreased N content at FTIR band characteristics β-sheets prove structural changes. At denaturation accompanied significant loss due dehydration decarbonylation reactions observed. Oscillatory measurements optimally hydrated describing network properties material two along temperature ramp 90 °C: 56–64 necessary trigger increases ratio gliadin total mass, determined reversed-phase high performance liquid chromatography (RP-HPLC). 79–81 complete occurs. confirms process showing shifts both steps.
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ژورنال
عنوان ژورنال: International Journal of Biological Macromolecules
سال: 2021
ISSN: ['1879-0003', '0141-8130']
DOI: https://doi.org/10.1016/j.ijbiomac.2021.01.008